Mouse Family With Sequence Similarity 135 Member B ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Family With Sequence Similarity 135, Member B in biofluid samples, such as tissue homogenates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . FAM135B present in samples reacts with an anti-Mouse FAM135B capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-FAM135B detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active FAM135B in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse FAM135B. Detection Target: Mouse Family With Sequence Similarity 135, Member B Reactivity: Mouse Range: 0.156-10ng/mL Sensitivity: 0.057ng/mL Storage Conditions: Store all components at 4┬░C This Mouse FAM135B activity assay is for the quantitative determination of Family With Sequence Similarity 135, Member B in samples including tissue homogenates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.